Unraveling the Composition and Behavior of Heterogeneous Lipid Nanodiscs by Mass Spectrometry
نویسندگان
چکیده
منابع مشابه
Visualization of lipid droplet composition by direct organelle mass spectrometry.
An expanding appreciation for the varied functions of neutral lipids in cellular organisms relies on a more detailed understanding of the mechanisms of lipid production and packaging into cytosolic lipid droplets (LDs). Conventional lipid profiling procedures involve the analysis of tissue extracts and consequently lack cellular or subcellular resolution. Here, we report an approach that combin...
متن کاملAnalysis of fatty acid composition of Withania coagulans fruits by gas chromatography/mass spectrometry
Background and objectives: Withania coagulans Dunal (Solanaceae) fruits are recommended to treat asthma, dyspepsia, biliousness, flatulent colic, liver complaints, intestinal infections, strangury, wounds and as diuretic, emetic and sedative agent in Indian traditional system of medicine. The objective of this study was to describe the systematic fatty acid composition...
متن کاملGlycoprotein analysis using mass spectrometry: unraveling the layers of complexity.
A glycoprotein exists as a heterogeneous mixture of forms due to differential glycosylation, each of which may confer different functionality and/or serve as a biochemical marker for disease. The complex structure of glycans make them a bioanalytical challenge requiring multiple mass spectrometry based approaches to gain different types of information. The following article will briefly describ...
متن کاملAnalyzing native membrane protein assembly in nanodiscs by combined non-covalent mass spectrometry and synthetic biology
Membrane proteins frequently assemble into higher order homo- or hetero-oligomers within their natural lipid environment. This complex formation can modulate their folding, activity as well as substrate selectivity. Non-disruptive methods avoiding critical steps, such as membrane disintegration, transfer into artificial environments or chemical modifications are therefore essential to analyze m...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2016
ISSN: 0003-2700,1520-6882
DOI: 10.1021/acs.analchem.6b00851